To investigate the capacity of anti-inflammatory cytokines and biological response modifiers (BRMs) to induce IL-1β, IL-6, TNF-α gene overexpression from mouse macrophages, we isolated the resident peritoneal macrophages from BALB/c mouse (8 week old) and incubated for 6 h with lipopolysaccaride (LPS) and Moschus moschiferus (MOMS) extracts. Analysis of inflammatory cytokines gene expression was carried out by RT-PCR amplification. Amplified PCR products were electrophoresed on 1.2% agarose gel, and the analysis (Ht) was used to 1D-density program.
1. LPS and MOMS extract treatments resulted in a significant decrease in IL-1β, IL-6, TNF-α mRNA expression level compared with the LPS treatment.
2. Among four sample of MOMS, Inhibitory effects of MOMS-A and MOMS-D for inflammatory cytokines gene expression were to be fine compared with the MOMS-B and MOMS-C.
According to the above data, because the anti- tumoral and anti-inflammatory response activities of macrophage are known to be dependent on the production of inflammatory cytokines (IL-1β, IL-6, TNF-α) by macrophages, we suggest that evaluations of BRM for the reduction of inflammatory cytokines production by macrophages are important for clinical application.
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